Kane has missed Tottenham’s previous two matches due to a hamstring injury, but trained with the rest of the team on Tuesday.
Manager Mauricio Pochettino is confident the England frontman will start against Los Blancos, while Dele Alli is also available after completing his three-match suspension.
Victor Wanyama is sidelined as he recovers from a knee injury, but long-term absentee Erik Lamela may be included in the match-day squad for the first time in over a year as he continues his rehabilitation from a hip problem.
Former Spurs star Gareth Bale is ruled out for Real Madrid, while Keylor Navas, Dani Carvajal and Raphael Varane are also unavailable.
Scroll through the gallery above to see how Tottenham could line up for their Champions League clash with Real Madrid.
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