Stefanos Tsitsipas was jeered by the US Open crowd after taking another lengthy bathroom break during his second-round win over Adrian Mannarino.
The Greek third seed had angered Andy Murray with his long trips to the loo in their marathon five-setter on Monday.
Tsitsipas looked to be cruising into round three when he took a two-set lead against Frenchman Mannarino.
Stefanos Tsitsipas was roundly booed by the US Open crowd after taking another loo break
The Greek star left Arthur Ashe for seven minutes after losing the third set on Wednesday night
But he was up to his old tricks again after dropping the third set, leaving the court for more than seven minutes.
The late-night crowd on Arthur Ashe made their feelings clear when he finally returned, but the fired-up world number three went on to complete a 6-3 6-4 6-7 (4) 6-0 victory.
Tsitsipas had enfuriated Sir Andy Murray by using the same tactics in their first-round thriller earlier this week. The former World No 1 tweeted on Thursday morning: ‘Did anything interesting happen overnight?’ in a dig at the Greek.
Afterwards, Tsitsipas insisted: ‘I haven’t done anything wrong, so I don’t understand. The people love the sport, they come to watch tennis. I have nothing against them. I love the fans.
‘But some people don’t understand. They haven’t played tennis at high level to understand how much effort and how much difficult it is to do what we are doing.
French outsider Adrian Mannarino’s momentum was scuppered and he lost the fourth 6-0
‘Sometimes we need a short break to do what we have to do.’
Clearly irked by criticism from Murray and some other players, Tsitsipas brought up a toilet break the Scot took after the fourth set of his victory in the final against Novak Djokovic in 2012.
When informed that break took less than three minutes, he replied: ‘OK. So three minutes more makes a difference?’
He concluded: ‘I’ve done everything the right way. If I haven’t, I should be penalised. I completely agree with that.
‘But as far as I know, you know, it is a necessity. It is a need when I’m out there playing, performing.’
Tsitsipas roars in defiance of the disapproving crowd after dominating in the fourth set
Another leak, this time in the roof, caused havoc with the scheduling inside the adjacent Louis Armstrong Stadium.
Play between Kevin Anderson and Diego Schwartzman was halted after the torrential rain in New York began blowing in sideways through openings under the roof.
The freak conditions forced the players off the soaking wet court while spectators had to shelter under umbrellas.
The match was initially interrupted for 35 minutes while the surface was dried but after a brief restart – during which 11th seed Schwartzman won the first set on a tie-break – the match was once again suspended.
The 23-year-old protested his innocence to the crowd after his antics irked them in New York
It was not the only leak to enrage, as a problem with the roof allowed rain to enter the court
They resumed following the Tsitsipas match on Ashe with Argentinian Schwartzman winning 7-6 (4) 6-3 6-4.
Angelique Kerber’s clash with Anhelina Kalinina, due on afterwards, had to be pushed back until Thursday.
Daniil Medvedev’s bid for an elusive first grand slam title continued to gain momentum as he brushed aside Dominik Koepfer.
The second seed, who was a finalist at Flushing Meadows in 2019, following up his first-round thrashing of Richard Gasquet with a 6-4 6-1 6-2 triumph inside two hours.
Britain’s Andy Murray was angered by Tsitsipas’s questionable toilet break in the first round
Medvedev said: ‘It was a great level from me – there were a few tight moments in the match that I managed to hold well, and I’m really happy to be through in less than two hours.’
Medvedev’s compatriot Andrey Rublev, the fifth seed, found the going tougher but still emerged as an ultimately comfortable 7-6 (2) 6-7 (5) 6-1 6-1 winner over Pedro Martinez of Spain.
In a significant upset, world number 117 Botic Van De Zandschulp surged past eighth seed Casper Ruud 3-6 6-4 6-3 6-4 to reach the third round of a grand slam for the first time.
Fifteenth seed Grigor Dimitrov retired with a foot injury as he trailed Australia’s Alexei Popyrin 7-6 (4) 7-6 (4) 4-0.