Twitter legal and policy chief Vijaya Gadde claimed the company will always enforce its rules, and does not offer “a blanket exception for the president or anyone else”.
Critics have repeatedly called for Trump’s Twitter account to be deleted after controversial tweets.
Last month Trump used the social media platform to call former White House staffer Omarosa Manigault, who has written a tell-all book criticising his record, “wacky” and a “dog”.
He has also repeatedly used Twitter to threaten foreign leaders.
In July, after Iranian President Rouhani said a US-Iran conflict would be the “mother of all wars”, Trump attacked the foreign leader.
He tweeted: “To Iranian President Rouhani.
“Never, ever threaten the United States again or you will suffer consequences the likes of which few throughout history have ever suffered before.”
On January 3 he used Twitter to warn North Korean leader Kim Jong-Un, saying: “I too have a nuclear button, but it is a much bigger and more powerful one than his, and my button works.”
Twitter’s terms and conditions explain the company prohibits: “Behaviour that crosses the line into abuse, including behaviour that harasses, intimidates or uses fear to silence.”
Trump has 54million followers on his personal Twitter account, @realDonaldTrump, which was setup in 2009.
He also has access to the official President of the United States account, which has 24million followers.
Twitter CEO Jack Dorsey has previously admitted he gets a notification on his phone every time Trump tweets.
When asked before if the platform would consider removing Trump’s account he said: “We have to balance it with the context that it’s in”.
“So my role is to ask questions and make sure we’re being impartial, and we’re upholding consistently our terms of service, including public interest.”
A number of prominent Trump supporters have had their Twitter accounts removed following allegations of misconduct.
These include far-right conspiracy theorist Alex Jones and provocative right-winger Milo Yiannopoulos.
Trump recently picked a fight with another online giant claiming Google is rigging the search results for the term “Trump news” against him.
Steve Grove, Director of Google’s News Lab, strongly denied the allegation.