The singer previously sent the Trump campaign two cease-and-desist letters in 2015 for its use of the band’s music.
A new letter, sent by his lawyer Dina LaPolt, said: “As we have made clear numerous times, Mr Trump is creating the false impression that our client has given his consent for the use of his music, and even that he endorses the presidency of Mr Trump.
“By using Livin’ On The Edge, without our client’s permission, Mr Trump is falsely implying that our client, once again, endorses his campaign and/or his presidency, as evidenced by actual confusion seen from the reactions of our client’s fans all over social media.”
Tyler took to Twitter to say the letter was not about his political beliefs, but about protecting copyright.
He wrote: “THIS IS NOT ABOUT DEMS VS. REPUB. I DO NOT LET ANYONE USE MY SONGS WITHOUT MY PERMISSION. MY MUSIC IS FOR CAUSES NOT FOR POLITICAL CAMPAIGNS OR RALLIES. PROTECTING COPYRIGHT AND SONGWRITERS IS WHAT I’VE BEEN FIGHTING FOR EVEN BEFORE THIS CURRENT ADMINISTRATION TOOK OFFICE.
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“THIS IS ONE OF THE REASONS WHY @JOEPERRY AND I HAVE BEEN PUSHING THE SENATE TO PASS THE MUSIC MODERNIZATION ACT. NO IS A COMPLETE SENTENCE.”
After Tyler’s cease-and-desist letters in 2015, Mr Trump tweeted: “Even though I have the legal right to use Steven Tyler’s song, he asked me not to. Have better one to take its place!
“Steven Tyler got more publicity on his song request than he’s gotten in ten years. Good for him!”