Hard-left MPs, who are all members of opposition leader Jean-Luc Mélenchon’s La France Insoumise (France Unbowed) party, submitted an amendment to parliament’s code of conduct stating “only the French flag and the United Nations flag” should be allowed to fly in the Chamber.
They said removing the EU’s blue and gold flag was “in line with the country’s historical political project of independence”.
But the amendment was rejected by an overwhelming majority of their peers.
And French President Mr Macron said: “At a time when some are attempting to turn France inwards… I would like you to know that I plan to officially recognise the EU flag and anthem at an EU summit next week.
“This way, no one will be able to remove the EU flag from the Chamber, nor from any other government building for that matter.”

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Speaking at a panel discussion at Frankfurt’s Goethe University, he added that he himself had chosen to play the EU anthem – the prelude to Beethoven’s Ode to Joy – at his victory rally in May.
The EU flag has been present in the hemicycle – the Chamber – for more than a decade.
It was first introduced by conservative Jean-Louis Debré, the then president of the National Assembly, in 2007, after several MPs deplored its absence from the Chamber.
Mr Macron has made no secret of his pro-EU stance, having published a joint plan with Germany for more EU integration in 2015.
This has continued throughout his political campaigning, and since becoming President Mr Macron has also pushed for more eurozone integration including the creation of a commissioner post responsible for the eurozone budget.
Last month the president set out his own vision for Europe where he also called for a common defence and immigration policy for EU members.