A tremor measuring 5.2 on the Richter scale hit at 8AM on Wednesday, August 30, six miles beneath the surface, according to the US Geological Survey.
The quake struck 51.7 miles southeast of Inarajan Village which is inhabited by some 2,300 people.
The extent of damage, mortality and injuries us still unknown.
The earthquake is the second in as many weeks after a smaller 4.2 tremor struck Guam on August 21.
The US National Weather Service said that there is no threat of a tsunami following the tremor.
Guam has been at the centre of many headlines over the last month after North Korean dictator Kim Jong-Un threatened to launch missiles into the waters of the small island amid increasing tensions with US President Donald Trump.
Tensions were increased earlier this week when missiles fired over Japan were supposedly practice for a North Korean attack on Guam.
The country’s Korean Central News agency quoted Kim Jong-Un as saying: “The current ballistic rocket launching drill like a real war is the first step of the military operation of the KPA in the Pacific and a meaningful prelude to containing Guam.”