The black Bell 47G is one of four seen pursuing Sean Connery’s 007 through the mountains in the 1967 film.
The 9ft model, which does not fly but could be made to, is the largest and last of the scaled-down choppers used in the film.
Specially built for Pinewood Studios by staff, it was “flown” using gantry cranes and arm extensions.
It is among 304 lots in an online automobilia sale on Sunday by Cheshire-based auctioneers H&H Classics.
The company’s Adam Sykes said: “This is a unique opportunity to purchase an important piece of James Bond memorabilia with impeccable provenance.”