Eighth-tier Marine defeated League Two Colchester on penalties on a day of multiple upsets in the FA Cup first round, where non-league sides Darlington, Stockport, King’s Lynn, Dagenham and Boreham Wood all beat EFL clubs.
Michael Howard was the hero for Merseyside-based Marine, a side in the Northern Premier League Division One North West, scoring the winning spot-kick to secure a 5-4 shootout win after Joshua Bohui had put his effort wide.
Anthony Miley had put the underdogs ahead midway through the first half but Harry Pell levelled after 64 minutes and, because there are no replays this season to ease fixture congestion, the tie went straight to extra-time.
Marine’s Adam Hughes converts his penalty during the shootout win over Colchester United
It was still 1-1 after 120 minutes and following six successful spot-kicks, Us substitute Bohui missed the target which allowed Howard to step up and secure an historic win for Marine.
Adam Campbell bagged a brace for National League North side Darlington, who claimed a 2-1 victory at League One Swindon.
Campbell put Darlington ahead just after the half-hour after intercepting a headed back-pass and stroking beyond Swindon goalkeeper Matej Kovar but the hosts levelled four minutes before half-time through Brett Pitman.
But Campbell would have what would prove to be the last word with a deflected strike on the hour as the Quakers, in the sixth tier of English football, reached the second round for the first time since 2006.
John Rooney and Alex Reid struck inside the opening quarter of an hour as National League promotion hopefuls Stockport won 2-1 at League One Rochdale.
The hosts pulled one back in the 22nd minute through Matthew Lund but were unable to find a leveller.
Sonny Carey came off the bench to score eight minutes from time as fifth-tier King’s Lynn reached the second round for the first time in their 10-year history by winning 1-0 at League Two Port Vale.
Boreham Wood advanced after a 4-3 shootout victory over Southend, who are rock-bottom of League Two and were knocked out of the cup as Jason Demetriou missed the decisive penalty, following a 3-3 draw over 120 minutes.
In the Saturday evening game, FC United of Manchester were brushed aside 5-1 at home to Doncaster, with Josh Sims scoring in either half and further goals coming from Fejiri Okenabirhie, Ben Whiteman and James Coppinger.
Josh Sims (right) celebrates after scoring Doncaster’s fifth against FC United of Manchester
League Two Stevenage avoided a setback against National League South Concorde after winning 5-4 on penalties following a 2-2 draw while Nigel Clough’s reign as Mansfield manager started with a 1-0 victory at Sunderland.
Gillingham came from two goals down to beat Woking 3-2, with Vadaine Oliver scoring the winner 11 minutes from time, while Charlie Kirk struck a quarter of an hour from the end as Crewe edged out Bolton by the same scoreline.
Brackley beat Bishop’s Stortford 3-2 on penalties after an entertaining 3-3 draw, in which Bishop’s Stortford twice came from two goals down, while Elliott Ronto’s goal helped Canvey Island defeat Banbury United 2-1.
Yeovil won 1-0 at Bromley courtesy of Gabriel Rogers’ strike in the last moments of extra-time, Scott Wilson’s brace helped Dagenham overcome Grimsby 3-1, while Luke Jephcott struck as Plymouth won 1-0 at Charlton.
Peterborough’s Siriki Dembele (far right) celebrates putting the Posh 1-0 up against Oxford
Jack Taylor’s strike gave League One table-toppers Peterborough a 2-1 triumph at Oxford while Jorge Grant and Anthony Scully each scored twice as Lincoln thrashed Forest Green 6-2.
Sean Raggett’s extra-time winner helped Portsmouth edge to a 3-2 success at Ipswich, who came back from 2-0, while Salford advanced after downing Hartlepool 2-0 in a game which also required an additional half-hour.
Bristol Rovers won 2-1 at Walsall thanks to first-half goals from Jack Baldwin and Brandon Hanlan while Tranmere overcame Accrington by the same scoreline, Peter Clarke bagging the winner for Rovers six minutes from time.
Bradford thumped Tonbridge Angels 7-0, Hull saw off Fleetwood 2-0, Newport edged out Leyton Orient 2-1, Exeter came from behind to triumph 2-1 over AFC Fylde, Cheltenham beat South Shields 3-1 and Shrewsbury won 2-0 at Cambridge.