Kyrgios has been heavily critical of the world No 1 this year, slamming his “cringeworthy” celebration in an explosive interview on the No Challenges Remaining podcast.
The Australian also thinks Djokovic has a “sick obsession” with wanting to be liked as much as Roger Federer.
During Queen’s last week, Kyrgios fumed about how Djokovic’s controversial antics on court never get highlighted as much as his.
In the latest episode, Kyrgios asked Denis Shapovalov if he chopped up Djokovic during their practice.
Shapovalov is practicing at Boogles in Stoke this week and he had a hit with Djokovic.
The young Canadian then posted a photo of them together on his Instagram page with the caption: “Great hit today with @djokernole.”
But Kyrgios commented with a sly dig at Djokovic.
“Did you chop him up,” the 23-year-old wrote.
Supporters were largely critical of Kyrgios’ decision to take a swipe at the 15-time Grand Slam winner.
“I don’t think @denis.shapovalov should delete THAT comment… just let everyone see how RUDE and how PATHETIC he(ky) is,” one fan wrote.
And another added: “If ATP would start giving trophy to the most idiotic player, @k1ngkyrg1os would be hands down winner everytime.”
While a further user wrote: “We don’t need to waste anymore energy on Nick. Move on with positivity.
“He is so negative and bitter. Needs to learn respect. Probably jealous.”
Kyrgios jibe came just days after he was fined £13,766 ($17,500) for his behaviour at Queen’s.
The world No 43 was punished for three counts of unsportsmanlike behaviour, which included calling an umpire a “nutter” and accusing a line judge of “rigging” a match.