With Barcelona already 2-0 up against Sevilla courtesy of Philippe Coutinho and Messi, things were looking good for Ernesto Valverde’s side.
But just after the quarter of an hour mark, Messi was involved in a nasty fall.
Having initially lost the ball, the Barcelona talisman attempted to win it back before he was clattered to the floor.
Messi hit the turf hard and in attempting to cushion his fall, his outstretched arm landed awkwardly.
HAS LIONEL MESSI BROKEN HIS ARM?
His lower arm, from the elbow down, appears to snap back causing the Argentine star to scream out.
The five-time Ballon d’Or winner looked to be in agony as he was dragged off the pitch by two members of medical staff.
After being off the pitch for 10 minutes receiving treatment it was decided he would play no further part in the game.
Messi headed down the tunnel with his shirt acting as a makeshift sling with former player Gary Lineker suggesting his arm could be broken.
WARNING: GRAPHIC PICTURES BELOW
“Sadly 15 minutes of Messi is all we’re going to get,” Lineker tweeted.
“Looks like he’s seriously damaged his arm…may be broken.”
Italian journalist Tancredi Palmeri also feared the worst for the forward.
“Messi out for serious injury!” he tweeted.
“His elbow turning as he puts down the arm falling, it seems it could be broken and in a serious way.”
Barcelona have since confirmed he will be out for three weeks with a fractured arm.
A Barcelona statement read: “Tests carried out on the first team player Leo Messi have confirmed that he has a fracture of the radial bone in his right arm.
“He will be out for approximately three weeks. #FuerzaLeo”
Messi will now miss their upcoming Champions League clash against Inter Milan on Wednesday night.
He will then be ruled out of the first El Clasico of the season when Real Madrid pay a visit next Sunday.
He is also likely to miss the return leg against Inter Milan on November 6, with Barca also playing Cultural Leonesa in the Copa del Rey and Rayo Vallecano in La Liga before that.