The US President is expected to announce his plan to move the American embassy to the holy city today. He is also expected to declare Jerusalem the capital of Israel.
But the move has led several Palestinian groups to call for three “days of wrath” starting on Wednesday in retaliation, according to the Israeli newspaper Haaretz.
The Israeli Federal Foreign Office responded by updating its travel advice to Israel and the Palestinian territories that there could be riots in the streets.
It warned: “Violent conflicts can not be ruled out.”
In Bethlehem, demonstrators burned pictures of Mr Trump on Tuesday night.
And international condemnation has poured in from Turkey and Iran, which warned the US leader the move could cause chaos in the Middle East.
However, Mr Trump appears to be pressing ahead.
Israeli police spokesman Micky Rosenfeld said on Wednesday security forces are always ready to respond to incidents.
Mr Trump plans to recognise Jerusalem, rather than Tel Aviv, as the Israeli capital, contrary to the fact it is not recognised as such by the international community or Palestine.
His announcement sparked fierce criticism from Turkish President Recep Tayyip Erdogan. He threatened to break relations with Israel.
Iran also blasted the US President’s decision as a sign of weakness.
Iran’s supreme leader Ayatollah Ali Khamenei said: “To declare Jerusalem the capital of occupied Palestine shows only the despair and inability to act (of the United States and Israel).
“Ultimately, however, Palestine will be liberated, and the Palestinians and the Islamic world will be victorious.”
Meanwhile, Israel’s intelligence minister Israel Katz warned the Palestinians against carrying out violent protests.
He said: “I suggest that not tensions will be cause and that they do not follow this path. Violent protests would be a big mistake by the Palestinian Authority.”
Israel conquered and later annexed the Arab-dominated eastern part of Jerusalem in 1967 during the Six-Day War. It claims all of Jerusalem as its indivisible capital. This claim is not recognised internationally. The Palestinians see their future capital in East Jerusalem.
Mr Katz said: “The American president is not intimidated by threats. He believes that Jerusalem must be Israel’s capital.”
But, a senior adviser to Palestinian President Mahmoud Abbas warned the US on Wednesday of unpredictable consequences of the announced recognition.
Abdallah al Frangi, Governor of Gaza, told Deutschlandfunk that America had put itself entirely on the side of Israel and the Middle East would be “turned upside down.”
He said the mediator role of the American president in the region is therefore over and branded the American plans an “act of violence”.
He feared that those affected will respond very strongly and that this “will be very unpleasant, even for the United States”.
He added: “I think the majority of the world community is against this move by the American president.”
Additional reporting by Monika Pallenberg