The European Council president, Poland’s former prime minister, slammed the country’s leadership, saying it was pursuing an isolationist policy.
European chiefs have been highly critical of the state’s ruling right-wing Law and Justice (PiS) party with a series of disputes over immigration.
And Mr Tusk used Twitter today to again meddle in the country’s affairs.
He tweeted: “Alarm! A vehement dispute with Ukraine, isolation in the European Union, departure from the rule of law and independent courts, attack on non-governmental sector and free media – PiS strategy or Kremlin’s plan?”
“Too similar to rest easy.”
On Saturday, the Polish ambassador was summoned to Kiev after a Ukranian official was denied entry to the eastern european state.
In response Prime Minister Beata Szydlo tweeted that Mr Tusk had “done nothing for Poland”.
She wrote: “Today, using his position to attack the Polish government, he is attacking Poland.”
Ms Szydlo has previously hit out at the EU “elites” accusing them of being out of touch with normal people.
The Polish government denies all charges from Brussels that it is undermining the rule of law or isolating Poland in Europe.
It insists it needs to overhaul Poland’s ineffective legal system and stand up for Poland’s interests in the EU.
On Wednesday, a majority of EU politicians demanded punishment for the eurosceptic government in Poland, saying it was undermining the rule of law and promoting intolerance.
Guy Verhofstadt has threatened financial sanctions on Poland if the country’s Government “continues to breach the rule of law and European values.”
That led to a spectacular row this week hen the PiS European Parliament delegation clashed with Mr Verhofstadt.
Polish MEP Michal Boni claimed the relationship between Poland and the bloc was now “destroyed”.
He told Hromadske International: “Over 20 years we have worked very hard to create a strong position for Poland among countries of the European Union.
“Now it’s destroyed. This is a problem and this is a problem not only for us.
“This is a problem for our colleagues and friends from this point of view because there is no leadership to talk with Brussels very strongly about our interest.”
In October, European Commissioner Elżbieta Bieńkowska warned Poland was an even bigger threat to the EU than Brexit.