JFK was shot dead by a sniper on November 22, 1963, in Texas in an incident that shook the world to its core.
Now, more than 50 years after the tragedy, people across the world will be able to access previously classified information that could lead to new discoveries and finally put some of the conspiracy theories to rest.
This is the final batch related to the assassination and the release contains more than 3,000 files, each of which could contain hundreds of documents.
The National Archives will be releasing the files later today where they will be available for download.
While no official release time has been revealed, The National Archives have indicated the files will be released on October 26, and the last time the National Archives released a set of JFK documents was on July 24 when they released them shortly after 7am Eastern Time (12.00 GMT).
Some people believe the release may be delayed while President Trump decides whether any of the files still need to remain classified if they pose a threat to national security.
Last weekend he tweeted: “Subject to the receipt of further information, I will be allowing, as President, the long blocked and classified JFK FILES to be opened.”
Kennedy assassination experts are not expecting any major bombshells, according to reports.
Gerald Posner, the author of ‘Case Closed: Lee Harvey Oswald and the Assassination of JFK’, told CNN he does not expect any major shocks.
He said: “There’s going to be no smoking gun in there.
“Anybody who thinks this is going to turn the case on its head and suddenly show that there were three or four shooters at Dealey Plaza it’s not the case.
“Oswald did it alone… but what the files are doing and why they’re important to come out is they fill in the history of the case and show us how the FBI and CIA repeatedly hid the evidence.”
Information about the activities of Lee Harvey Oswald, who is widely believed to have been Kennedy’s killer, in the run-up to the tragedy may shed new light on the investigation.
Oswald, a former US Marine, was arrested on the day of JFK’s assassination and charged with the President’s murder, despite denying he was guilty.
He was gunned down by nightclub owner Jack Ruby while in police custody two days later.
Mr Posner told USA Today that the new information may prove embarrassing to some CIA agents after he plot to kill Kennedy became the most powerful conspiracy theory in American history.
He said: “There may not be deep, dark secrets in there, but the release could be embarrassing to people who were involved.
“You have to remember that Mexico City in the 1960s was a hodgepodge of intrigue where everyone was spying on everyone else.
“There may be people who were informing to the CIA at the time who have moved on to careers in politics and business, and the revelation that they were informing will be embarrassing to them.”