Donald Trump criticised Pakistan for allegedly harbouring “agents of chaos” and providing safe havens to militant groups waging an insurgency against the US-backed government in Kabul.
And he said the situation “must change immediately” as US Secretary of State Rex Tillerson warned reporters that Washington would consider cutting aid to Pakistan if things did not improve.
But the comments have been met with fury from the government, with the National Assembly now unanimously passing a resolution calling on the state to cut ground and air supply lines to the US-led NATO mission.
And hundreds of people have taken to the streets in huge protests, with many waving placards and banners – but some burned posters of Mr Trump’s face in anger over his comments.
Speaking before the assembly, Pakistani Foreign Minister Khawaja Asif also urged the government to consider postponing any visits by US delegations to Pakistan or by Pakistani officials to the US.
Washington also has no choice but to use Pakistani roads to resupply its troops in landlocked Afghanistan.
And US officials worry that if Pakistan becomes an active foe, it could further destabilise Afghanistan and endanger soldiers.
Mr Asif said: “Afghanistan, the US and its allies should close their borders to leaders of terrorist, militant groups carrying out acts of terrorism against Pakistan.”
The resolution, which was adopted on Wednesday, refers to remarks made in Mr Trump’s speech earlier this month announcing a new US strategy for the war in Afghanistan.
Hundreds of people have rallied against the remarks, however, saying they ignore Pakistan’s sacrifices in its war on terror.
It is estimated there have been 70,000 Pakistani casualties in militant attacks since it joined the war on terror after the 9/11, attacks.
But Pakistan, which borders Afghanistan, denies that it offers sanctuary to any armed groups, including the Afghan Taliban and the Haqqani Network.
A statement from the National Assembly read: “The National Assembly regards President Trump’s and General Nicholson’s statements on Pakistan as hostile and threatening.”